生物制造 经典文献导读|2017年11月08日

Human induced pluripotent stem cell-derivedfiber-shaped cardiac tissue on achip

在芯片上的人类诱导多能干细胞衍生的纤维形心脏组织

Y. Morimoto,S. Mori,F. Sakaiab and S. Takeuchi*. Lab Chip,2016, 16, 2295

http://pubs.rsc.org/en/content/articlelanding/2016/lc/c6lc00422a#!divAbstract

Abstract

We propose a method for the production of a fiber-shaped three-dimensional (3D)cellular construct ofhuman induced pluripotent stemcell-derived cardiomyocytes (hiPS-CMs) for the quantification of thecontractile force. By culturing the cardiomyocytes in a patterned hydrogelstructure with fixed edges, wesucceededin fabricating hiPS-CM fibers with aligned cardiomyocytes. The fiber generatedcontractile forcealong the fiber direction due tothe hiPS-CM alignment, and we were able to measure its contractile forceaccurately.Furthermore, to demonstrate the drug reactivity of hiPS-CM fibers, the changesin the contractile frequency and force following treatment with isoproterenoland propranolol were observed. We believethathiPS-CM fibers will be a useful tool for pharmacokinetic analyses during drugdevelopment.

(导读：夏圣悦)心肌细胞的自主搏动是心肌细胞的特异性功能，本文设计了一种可以使心肌细胞定向对齐生成纤维心脏组织的微流控芯片。在这个芯片中，纤维会产生沿着对齐方向的定向收缩。然后对这种对齐的心脏纤维组织的收缩力进行了定量计算和与块状心脏组织的对比，并验证了这种定向纤维心脏组织的药物反应性。

Light-triggered in vivoactivation of adhesive peptides regulates cell adhesion, inflammation andvascularization of biomaterials

基于光触发机制的体内活化生物材料内部粘附肽链以调控细胞粘附、抗炎及血管化活动

Lee, Ted T. García, José R. Paez,Julieta I. Singh, Ankur Phelps, Edward A. Weis, Simone Shafiq, Zahid Shekaran,Asha del Campo, Aránzazu García, Andrés J.*. Nature Materials. 2014 Dec 15;14(3).

http://www.nature.com/doifinder/10.1038/nmat4157

Abstract

Materialsengineered to elicit targeted cellular responses in regenerative medicine mustdisplay bioligands with precise spatial and temporal control. Although materialswith temporally regulated presentation of bioadhesive ligands using externaltriggers, such as light and electric fields, have recently been realized forcells in culture, the impact of in vivo temporal ligand presentation oncell-material responses is unknown. Here, we present a general strategy totemporally and spatially control the in vivo presentation of bioligands usingcell-adhesive peptides with a protecting group that can be easily removed viatransdermal light exposure to render the peptide fully active. We demonstratethat non-invasive, transdermal time-regulated activation of cell-adhesive RGDpeptide on implanted biomaterials regulates in vivo cell adhesion,inflammation, fibrous encapsulation, and vascularization of the material. Thiswork shows that triggered in vivo presentation of bioligands can be harnessedto direct tissue reparative responses associated with implanted biomaterials.

（导读：姚弘毅）当前最具影响力的将光去屏蔽固化生物水凝胶用于体内试验的文献。通过351nm紫外光低强度经皮照射15分钟，将材料中的DMNPB基团去除，从而引发水凝胶自体聚合及细胞粘附肽链RGD的活化，最终在小鼠体内相应区域细胞完成粘附、抗炎、血管化等行为。本文充分展示了光调控水凝胶在生物医用领域广阔的应用前景。