Human induced pluripotent stem cell-derivedfiber-shaped cardiac tissue on achip
Y. Morimoto,S. Mori,F. Sakaiab and S. Takeuchi*. Lab Chip,2016, 16, 2295
We propose a method for the production of a fiber-shaped three-dimensional (3D)cellular construct ofhuman induced pluripotent stemcell-derived cardiomyocytes (hiPS-CMs) for the quantification of thecontractile force. By culturing the cardiomyocytes in a patterned hydrogelstructure with fixed edges, wesucceededin fabricating hiPS-CM fibers with aligned cardiomyocytes. The fiber generatedcontractile forcealong the fiber direction due tothe hiPS-CM alignment, and we were able to measure its contractile forceaccurately.Furthermore, to demonstrate the drug reactivity of hiPS-CM fibers, the changesin the contractile frequency and force following treatment with isoproterenoland propranolol were observed. We believethathiPS-CM fibers will be a useful tool for pharmacokinetic analyses during drugdevelopment.
Light-triggered in vivoactivation of adhesive peptides regulates cell adhesion, inflammation andvascularization of biomaterials
Lee, Ted T. García, José R. Paez,Julieta I. Singh, Ankur Phelps, Edward A. Weis, Simone Shafiq, Zahid Shekaran,Asha del Campo, Aránzazu García, Andrés J.*. Nature Materials. 2014 Dec 15;14(3).
Materialsengineered to elicit targeted cellular responses in regenerative medicine mustdisplay bioligands with precise spatial and temporal control. Although materialswith temporally regulated presentation of bioadhesive ligands using externaltriggers, such as light and electric fields, have recently been realized forcells in culture, the impact of in vivo temporal ligand presentation oncell-material responses is unknown. Here, we present a general strategy totemporally and spatially control the in vivo presentation of bioligands usingcell-adhesive peptides with a protecting group that can be easily removed viatransdermal light exposure to render the peptide fully active. We demonstratethat non-invasive, transdermal time-regulated activation of cell-adhesive RGDpeptide on implanted biomaterials regulates in vivo cell adhesion,inflammation, fibrous encapsulation, and vascularization of the material. Thiswork shows that triggered in vivo presentation of bioligands can be harnessedto direct tissue reparative responses associated with implanted biomaterials.